How to Reverse Sex Using Silver Thiosulphate

[Naycha]

How to Reverse Sex Using Silver Thiosulphate

Just a quick explanation of how to reverse the sex of a female Cannabis plant using Silver Thiosulphate. The reason for reversing sex is to induce fertile male flowers for the purpose of making feminised seed. The pollen produced will be used to pollinate an untreated female plant.

All you need for this technique is STS solution, 2 female plants, and a microlitre syringe




How to prepare STS solution

Prepare a 0.1 M Sodium Thiosuphate (STS) stock solution by dissolving 1.58 g of Sodium Thiosulphate Anhydrous (2.47g if using Sodium Thiosulphate Pentahydrate) into 100 ml of water. Prepare a 0.1 M Silver Nitrate stock solution by dissolving 1.7 g of Silver Nitrate into 100 ml of water. Store the stock solutions in the dark until needed to prepare the STS.

In general, the STS is prepared with a molar ratio between silver and thiosulphate of 1:4, respectively. Nearly all of the silver present in the solution is in the form of
[Ag(S₂O₃)₂]^3-, the active complex for ethylene effect inhibition.

Prepare a 0.02 M STS by slowly pouring 20 ml of 0.1 M silver nitrate stock solution into 80 ml of 0.1 M sodium thiosulfate stock solution while stirring. The STS can be stored in the refrigerator for up to a month. However, preparation of the STS just prior to use is recommended.


Once you have prepared the STS solution you can draw a measured amount into the microlitre syringe. The droplet in the photo is about 5 microlitres. In this instance I will be using 11 microlitres at a time once a day for 7 days.

As mentioned above I will be using a total of 77 microlitres of STS applied over 7 days. The first treatment will be applied just prior to the 1st night of the flowering schedule (in this example I am transplanting from a 40mm rockwool cube into soil and moving the plant outside). In this technique the STS is applied to the apical meristem only.

I will update this thread as the plant develops. All comments appreciated and I will try to answer any questions if I am able to. Edited June 13, 2020 by Naycha

[Frank Reynolds]

Very detailed as usual nay, gonna be fun to follow a long.

[GreatSouthernMan]

that needle looks nasty

[MongyMan]

Good stuff Nay

 

Just wondering why 7 applications though? Colloidal silver will need multiple applications but STS only needs 1 from my exp.

[Naycha]

Good stuff Nay

 

Just wondering why 7 applications though? Colloidal silver will need multiple applications but STS only needs 1 from my exp.

Cheers Mongy

 

I'm only doing 7 applications because it's the method I started out with and I have been too lazy to try another method. I would like to try other methods though. I am fond of using a micro droplet because it is easy to measure and adjust, it would certainly be more simple if I could use one application only.

[MongyMan]

I just use a spray bottle to apply STS but using micro drops is a more accurate way though and definitely interesting. Have you tried micro drop application just once?

[Naycha]

No I haven't tried it yet but it is on the list. Also considering trialling stem injection out of interest.

[MongyMan]

Cool will be interesting to see the results and @ the image of your plants on the road to hard core mainlining addiction to STS

[Naycha]

Cool will be interesting to see the results and @ the image of your plants on the road to hard core mainlining addiction to STS

it's a slippery slope indeed.

 

This is a few days after the last treatment. The tip has blackened and shrivelled and I don't expect it to recover (not mine, the plant's).

 

[buzzer]

The Tiresias you get from the hydro shop should work the same right? anyone here used it?

 

I know making your own is heaps cheaper. But I would rather just buy it in and spray it on. And a 50 buck outlay to make hundreds of feme seeds seems pretty worth it.

 

I've got 2 green crack autos that I wanna breed so I'm gonna have to turn 1

 

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