back-X Ineffective in selecting mj heterosis

[syk613]

I am not sure WC, but if a plant with 2x rec. gene is breed with others that contain the dominant form of the gene then there should be no reason the usual rules for genetic heretability don't apply ie rec/rec, rec/dom or dom/dom genotypes and ratios depend on the make up of the genotype of the plants that lead to the new plant.

But I don't have the knowledge about breeding mj or plants to give you any real info, but i will post it if i see anything.

[syk613]

Pipeman: Nov 6 2003, 05:37 PM

 

I am interested in how growing conditions affect THC/CBD content of your buds. Like does CO2 just increase yield or does it increase potency as well? Also I've read the humidity levels late in your grow can affect what kind of high your buds give.

@pipeman

i've had a quick look but I couldn't find much of interest, however I was looking for obsevational and plant chemistry analysis work done on the plants at the end of a grow, like the UK mj thread in this forum and in fact this was about the best info I could find to answer your question even if it is a fairly generalized explanation(the last sentence about compostion of THC. This paper could also provide a starting point to find more info and If I can get the whole reference online I will PM it to you as I don't think I can post it for copyright reasons.

Instead of this approach, after rereading your question, I may try to look for a gene function analysis that analyses the reaction of certain THC genes, and many others in mj, to a particular stimulus ie CO2, humidity etc and if the synthase for 9-THC and CBD or genes invovled in other crucil areas are upregulated ie more of the right proteins (or synthase or enzyme all the same thing essentially) are produced and these will move around the cell looking for the precursor molecules to THC in order to dutifully produce 9-THC for our entertainment. Ok so what does this mean? If one stimulus eg high humidity sets the mj to produce THC but not CBD this will show up in a gene function analysis and because this is a fairly new technol in this field and it is relatively easy to use, reliable and provides a shit load of info a lot of institutions are using microarray technology to analyse many 100s of genes in a day and it is considered a v powerful tech to find out what happens at the genetic level for a certain stimulus, I'm much more likely to find something very recentand it will talk about impt previous work also.

Cheers mate I hope this helps and it doesn't trip you out, I'll have a bit more of a look and I will also try to post a better answer (if there is one to both questions)to WCs question also but as I said I only had a quick look. Soz about the length I tend to ramble... a bit

Edited November 8, 2003 by syk613

[pipeman]

hey thanks syk I'll look forward to any studies like this you can find.

[syk613]

Justinova, Z; Tanda, G; Redhi, GH, et al.

 

Self-administration of Delta(9)-tetrahydrocannabinol (THC) by drug naive squirrel monkeys

PSYCHOPHARMACOLOGY, 169 (2): 135-140 SEP 2003.

 

 

I'll have to have a closer look at this expt.

[syk613]

@pipeman I found this in a different database, unfortunently it is not hydro or even smokable mj but it may be of interest to you or others.

 

 

Authors: Kadar, I; Szucs, ST

Title: Effect of mineral fertilisation on the yield of fibre hemp (Cannabis sativa L.)

Source: NOVENYTERMELES, 52 (2): 217-228 APR 2003

Keywords: mineral fertilisation; fibre hemp; yield; quality; soil analysis

KeyWords Plus: CALCAREOUS CHERNOZEM SOIL

Abstract:

The effect of various N, P and K supply levels and their combinations on the development, yield and quality of fibre hemp (variety Kompolti) was investigated in the 16(th) year of a long-term mineral fertilisation experiment on loamy chernozem soil with lime deposits. The soil of the growing site contained 3% humus, 5% CaCO3 and 20 % clay in the ploughed layer, and was classified as poorly supplied with P and Zn and moderately well with N and K. The experiment was set up with 4Nx4Px4K=64 treatments in 2 replications, giving a total of 128 plots. The mineral fertilisers were applied in the form of calcium ammonium nitrate containing 25% N, 18% superphosphate and 50% potassium chloride. The groundwater was at a depth of 13-15 in and the area was prone to drought. The main results were as follows:

1. The NxP interactions were dominant during the first two months of fibre hemp development and the NxK interactions during the second half of the vegetation period. Growth maxima were observed between May 30(th) and June 13(th), when the height of the plant stand increased by 78 cm in the course of 14 days, i.e. by 5.6 cm a day, as the result of plentiful NPK supplies.

 

2. The air-dry shoot mass was 0.6-1.3 t at the end of May, 3.5-8.0 t on June 26(th) and 7-12 t at stem harvest on August 11(th), while the fresh mass of the green stems was 25-41 t per hectare, depending on the treatment. Optimum supply levels were found to be 100 kg/ha/year N, 150 mg/kg ammonium lactate (AL)-soluble P2O5 and 250-300 mg . kg(-1) AL-K2O. These data could serve as guidelines for the Hungarian fertiliser extension service.

 

3. N fertilisation reduced the plant number, increased the length, thickness and fibre yield of the stems and led to a deterioration in fibre quality. Good quality was obtained on plots given no N for 16 years and satisfactory quality in treatments given 100-200 kg . ha(-1) . year(-1) N, while on plots fertilised with 300 kg . ha(-1) . year(-1) the quality was not suitable for use by the textile industry. After P fertilisation there was a drastic decline of almost 20 % in the plant number and in the % of crush, and a rise of 2 % in the fibre content of the crude stems. K fertilisation had a positive effect on this soil, which was only moderately supplied with K: there was an increase in the length and thickness of the stems, and a 0.4 t . ha(-1) increase in the fibre yield.

 

4. Among the treatment combinations, the following minimum-maximum values were recorded in the experiment: plant density 160-261 plants . m(-2), technical length of the stem 180-250 cm, stem thickness 4-8 mm, fibre yield 2.0-3.0 t . ha(-1).

Reprints: KADAR I,HUNGARIAN ACAD SCI,RES INST SOIL SCI & AGR CHEM; HUNGARY.

 

Research

Institutions: Hungarian Acad Sci, Res Inst Soil Sci & Agr Chem, Budapest, Hungary.

Hungarian Hemp Ind Trust, Hungarohemp, Szeged, Hungary.

[syk613]

Totally unrelated to the thread but I found it interesting even if it is a bit over my head hmm I wonder if the full text has pics?

 

Authors: Kim, ES; Mahlberg, PG

Title: Secretory vesicle formation in the secretory cavity of glandular trichomes of Cannabis sativa L. (Cannabaceae)

Source: MOLECULES AND CELLS, 15 (3): 387-395 JUN 30 2003

Keywords: basilar wall; cannabaceae; Cannabis; cryofixation; gland; secretory cavity

KeyWords Plus: ELECTRON-MICROSCOPY; HAIRS

Abstract:

The disc cell wall facing the secretory cavity in lipophilic glands of Cannabis was studied for origin and distribution of hyaline areas, secretory vesicles, fibrillar matrix and particulate material. Secretions evident as light areas in the disc cell cytoplasm pass through modified regions in the plasma membrane and appear as hyaline areas in the cell wall. Hyaline areas, surrounded with a filamentous outline, accumulate near the wall surface facing the secretory cavity where they fuse to form enlarged hyaline areas. Fibrillar matrix is related to and may originate from the dense outer layer of the plasma membrane. This matrix becomes distributed throughout the wall material and contributes in part to the composition of the surface feature of secretory vesicles. Thickening of the cell wall is associated with secretions from the disc cells that facilitates movement of hyaline areas, fibrillar matrix and other possible secretions through the wall to form secretory vesicles and intervesicular materials in the secretory cavity. The outer wall of disc cells in aggregate forms the basilar wall surface of the secretory cavity which facilitates the organization of secretory vesicles that fill the secretory cavity.

Reprints: KIM ES,KONKUK UNIV,DEPT BIOL; SEOUL 143701, SOUTH KOREA.

kimes@konkuk.ac.kr

Research

Institutions: Konkuk Univ, Dept Biol, Seoul 143701, South Korea.

Indiana Univ, Dept Biol, Bloomington, IN 47405 USA

[pipeman]

I don't think those articles told us anything meaningful, but, research into hemp could be useful for us pot growers. As conditions to make a better hemp plant applied in reverse could be good for pot plants. Know what I mean?

[white_cluster]

Hey Syk,

 

I wanna see some work by E. small on the breeding of strains for specific medical conditions, eg his work producing chemo.

 

find some and I'll love you forever.

[Guest IMPOSTORsyk613NON-MEMBER]

@ all members and guests: Any1with requests similar to those of WC and pipeman, for scientific literature on a specific subject (esp. med users), can be posted here (or a new thread and I will reply to indicate I have read the post) and I would be more than happy to have a look, maybe read this post to WC (below) for info to make it easier on me. Cheers

 

 

@ pipeman I don't have the horticultural knowledge to give an educated opinion on the 1st article (in jodas voice:A jedi we need, yes brurrr) experienced soil growers may be able to interpret the abstract and find it interesting enough to get the full version (maybe 10+ pages), it won't help with relative THC levels but perhaps it will with optimum growth conditions which should lead to more yield and more THC. It is after all a study on sativa's, even if the variety is not cultivated in Holland, and so genetically they should be fairly similar (the diffrerence between a chimp and us is about 1% in terms of DNA)

The second one I just thought was interesting and would interest others after reading the posts about magnifying glasses and the examination of buds It might, also, help members in the community to better understand he anatomy(if that is the right term for flora) of trichomes, personally I know sweet f*ck all about it.

I'm still looking though the articles and the other night was not a very meaningful search given the number of beers I'd had, and was more a general look, I am still to do a specific search with x-references for either obsevational evidence or studies examing causation in respect to your question and I will try to find something a little more practical but I had a hangover on sunday and the beach was alot more appealing than a billy and the computer screen.

 

 

@White Cluster

Ah shocks WC your gonna make me blush. But seriously I'd be more than happy to have a look for you especially if it is not just a general query and you have an authors name or the name of an article/published scientiic literature then it makes it a lot easier for me. If it is there I should be able to find it in a matter of minutes and if I can get a fulltext version then I will PM it to you but if not there may be an email addy so you can request an e-version of the article.So if you can reply with some more info on E. Small and his work: a title, date (approx),the journal it was published in or anything else you have(like co-authors) it will make it a lot easier

Oh also his full name would be helpful

breeding of strains for specific medical conditions, eg his work producing chemo.

By this do you mean chemotherapy? or do you mean breeding plants that contain specific chemicals eg THC that may be trialed for a med condition? or something else? IF it is a question about anti cancer therapy then fire away mate, I have 1st hand hand knowledge of these agents especially the agents that kill cells by directly damaging DNA. Cheers mate..

[syk613]

Yeah I forgot to register, thats what happens when you wake up at 4 am and start a long rambling reply