Cannabinoids Inhibit Glioma Cell Invasion

[CannabisMan]

Cannabinoids, the active components of Cannabis sativa L. and their derivatives, inhibit tumor growth in laboratory animals by inducing apoptosis of tumor cells and impairing tumor angiogenesis. It has also been reported that these compounds inhibit tumor cell spreading, but the molecular targets of this cannabinoid action remain elusive. Here, we evaluated the effect of cannabinoids on matrix metalloproteinase (MMP) expression and its effect on tumor cell invasion. Local administration of {Delta}9-tetrahydrocannabinol (THC), the major active ingredient of cannabis, down-regulated MMP-2 expression in gliomas generated in mice, as determined by Western blot, immunofluorescence, and real-time quantitative PCR analyses. This cannabinoid-induced inhibition of MMP-2 expression in gliomas (a) was MMP-2–selective, as levels of other MMP family members were unaffected; ( was mimicked by JWH-133, a CB2 cannabinoid receptor–selective agonist that is devoid of psychoactive side effects; © was abrogated by fumonisin B1, a selective inhibitor of ceramide biosynthesis; and (d) was also evident in two patients with recurrent glioblastoma multiforme. THC inhibited MMP-2 expression and cell invasion in cultured glioma cells. Manipulation of MMP-2 expression by RNA interference and cDNA overexpression experiments proved that down-regulation of this MMP plays a critical role in THC-mediated inhibition of cell invasion. Cannabinoid-induced inhibition of MMP-2 expression and cell invasion was prevented by blocking ceramide biosynthesis and by knocking-down the expression of the stress protein p8. As MMP-2 up-regulation is associated with high progression and poor prognosis of gliomas and many other tumors, MMP-2 down-regulation constitutes a new hallmark of cannabinoid antitumoral activity. [Cancer Res 2008;68(6):1945–52]

 

 

Cancer Research 68, 1945, March 15, 2008. doi: 10.1158/0008-5472.CAN-07-5176

© 2008 American Association for Cancer Research

 

 

http://cancerres.aacrjournals.org/cgi/cont...ourcetype=HWCIT

[Radic]

gotta love every word a dat

 

check dis

from Joan Bello

http://www.benefitsofmarijuana.com/ask/index.php

Clinical Studies - not reported!

Marijuana helps to prevent cancer.

Tests show that marijuana actually prevents cancer! for Breast Cancer, Brain Cancer, and now possibly Skin Cancer

– specific marijuana compounds have cured cancer!

The diabolic fact: in the 60’s, a government study in

New Mexico proved marijuana prevented cancer.

The Lynn Pearson Study was hidden for nearly 40 years by the government.

Ten years ago, it was found.

Modern science shows these same wonderful results. But it is still not making headlines,

not on radio

or TV.

But the truth can’t be hidden.

Clinical studies are all over the internet.

While most Americans don’t read professional journals,

most of us do read the truth about marijuana on the internet.

 

*A study published in the American Journal of Epidemiology in 1999,

reported that lifetime use of cannabis was associated with a reduced risk of adult,

non-Hodgkin’s lymphoma. *2004 issue of the Journal of Pharmacology and Experimental Therapeutics:

administration of cannabidiol (CBD) (a marijuana compound) significantly inhibited the growth of

subcutaneously glioma cells. (THAT’S BRAIN CANCER)

“In conclusion, CBD was able to produce a significant antitumor activity both in vitro and in vivo,

suggesting a possible application of CBD as an antineoplastic agent (an agent that inhibits the growth of malignant cells.)”

*Last year, Pacif. Med. Research Inst.- “administration of THC on human glioblastoma multiforme cell lines

decreased the proliferation of malignant cells

and induced apoptosis (programmed cell death).”

*Cannabidiol as a novel inhibitor of Id-1 gene expression in aggressive breast cancer cells. Mol Cancer Ther, 2007,

Nov; 6(11):2921-7. PMID: 18025276 [PubMed indexed for MEDLINE]

*Cannabinoids selectively inhibit proliferation and induce death of cultured human glioblastoma multiforme cells. J

Neurooncol. 2005 Aug;74(1):31-40. PMID: 16078104 [PubMed - indexed for MEDLINE]

 

BTW: 94 studies on Brain Cancer and Marijuana (Cannabis)

can be found on PubMed – the government’s own Medical database.

forward ever

free cannabis

heal the people

heal the land

Edited July 22, 2009 by Radic

[luciddreaming]

I feel like walking up to Krudd at his next press conference and yelling "Why are you letting people with cancer die when Cannabis has been proven over and over to inhibit the growth of Cancer cells and kill Cancer cells!?" Edited July 23, 2009 by luciddreaming

[Crunchy]

oh snap! yet another weapon of information to add to my arsenal